| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 626-925 / 925. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Nucleus membrane, Nucleus, nuclear pore, Chromosome, centromere, kinetochore. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P57740 Human | Q8BH74 Mouse | P52590 Rat |
| Alternative names: | 107 kDa nucleoporin NU107_HUMAN Nuclear pore complex protein Nup107 Nucleoporin 107kDa Nucleoporin Nup107 NUP107 NUP84 NUP84, yeast, homolog of OTTHUMP00000240604 OTTHUMP00000240606 |
|
Fig1:
Western blot analysis of Nup107 on different lysates with Rabbit anti-Nup107 antibody (HA500521) at 1/1,000 dilution. Lane 1: HeLa cell lysate(20 µg/Lane) Lane 2: HepG2 cell lysate(20 µg/Lane) Lane 3: RAW264.7 cell lysate(20 µg/Lane) Lane 4: NIH/3T3 cell lysate(20 µg/Lane) Lane 5: PC-12 cell lysate(20 µg/Lane) Lane 6: mouse testis tissue lysate(30 µg/Lane) Lane 7: rat testis tissue lysate(30 µg/Lane) Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500521, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 106 kDa Observed band size: 106 kDa |