Nup107 Rabbit Polyclonal Antibody
cat.: HA500521
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 106 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 626-925 / 925. |
| Positive control: | Hela cell lysate, 293T cell lysate, HepG2 cell lysate, Jurkat cell lysate, PC-3M cell lysate, A549 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, mouse testis tissue lysate. |
| Subcellular location: | Nucleus membrane, Nucleus, nuclear pore, Chromosome, centromere, kinetochore. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P57740 Human | Q8BH74 Mouse | P52590 Rat |
| Alternative names: | 107 kDa nucleoporin NU107_HUMAN Nuclear pore complex protein Nup107 Nucleoporin 107kDa Nucleoporin Nup107 NUP107 NUP84 NUP84, yeast, homolog of OTTHUMP00000240604 OTTHUMP00000240606 |
Images
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Fig1:
Western blot analysis of Nup107 on different lysates with Rabbit anti-Nup107 antibody (HA500521) at 1/1,000 dilution. Lane 1: Hela cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: PC-3M cell lysate (20 µg/Lane) Lane 6: A549 cell lysate (20 µg/Lane) Lane 7: MCF-7 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: RAW264.7 cell lysate (20 µg/Lane) Lane 10: NIH/3T3 cell lysate (20 µg/Lane) Lane 11: Mouse testis tissue lysate (30 µg/Lane) Predicted band size: 106 kDa Observed band size: 106 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500521) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.