Nup107 Rabbit Polyclonal Antibody
cat.: HA500521
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 106 kDa
Isotype: IgG
Immunogen: Recombinant protein within human aa 626-925 / 925.
Positive control: HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate.
Subcellular location: Nucleus membrane, Nucleus, nuclear pore, Chromosome, centromere, kinetochore.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P57740 Human | Q8BH74 Mouse | P52590 Rat
Alternative names: 107 kDa nucleoporin NU107_HUMAN Nuclear pore complex protein Nup107 Nucleoporin 107kDa Nucleoporin Nup107 NUP107 NUP84 NUP84, yeast, homolog of OTTHUMP00000240604 OTTHUMP00000240606
Images
HA500521_1.jpg Fig1: Western blot analysis of Nup107 on different lysates with Rabbit anti-Nup107 antibody (HA500521) at 1/1,000 dilution.

Lane 1: HeLa cell lysate(20 µg/Lane)
Lane 2: HepG2 cell lysate(20 µg/Lane)
Lane 3: RAW264.7 cell lysate(20 µg/Lane)
Lane 4: NIH/3T3 cell lysate(20 µg/Lane)
Lane 5: PC-12 cell lysate(20 µg/Lane)
Lane 6: mouse testis tissue lysate(30 µg/Lane)
Lane 7: rat testis tissue lysate(30 µg/Lane)

Exposure time: 4 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500521, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 106 kDa
Observed band size: 106 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.