Dicer Rabbit Polyclonal Antibody
cat.: HA500522
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 218 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human aa 701-1,100 / 1,922. |
| Positive control: | MCF-7 cell lysate, 293 cell lysate, Daudi cell lysate, MCF-7, C2C12. |
| Subcellular location: | Cytoplasm, perinuclear region. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 10ug/mL 1ug/mL |
| Uniprot #: | SwissProt: Q9UPY3 Human | Q8R418 Mouse |
| Alternative names: | DCR DCR1 Dicer 1 ribonuclease III Dicer 1 ribonuclease type III Dicer DICER_HUMAN DICER1 Double-strand-specific ribonuclease Endoribonuclease Dicer Helicase MOI Helicase with RNase motif HERNA KIAA0928 MNG1 RMSE2 |
Images
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Fig1:
Western blot analysis of Dicer on different lysates with Rabbit anti-Dicer antibody (HA500522) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 3: Daudi cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 218 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500522) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of MCF-7 cells labeling Dicer with Rabbit anti-Dicer antibody (HA500522) at 10ug/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Dicer antibody (HA500522) at 10ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of C2C12 cells labeling Dicer. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500522, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.