Cofilin Rabbit Polyclonal Antibody
cat.: HA500523
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cofilin aa 117-166 / 166. |
| Positive control: | Hela cell lysate, HEK-293 cell lysate, MCF-7 cell lysate, MDA-MB-468 cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, Jurkat cell lysate, COS-1 cell lysate, VERO cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Neuro-2a cell lysate, NIH/3T3, Hela. |
| Subcellular location: | Nucleus matrix, Cytoplasm, Cytoskeleton, Cell projection. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:200-1:1,000 |
| Uniprot #: | SwissProt: P23528 Human | P18760 Mouse | P45592 Rat |
| Alternative names: | 18 kDa phosphoprotein CFL 1 CFL CFL1 COF1_HUMAN Cofilin 1 Cofilin 1 non muscle Cofilin Cofilin non muscle isoform Cofilin-1 epididymis secretory protein Li 15 HEL-S-15 non-muscle isoform p18 |
Images
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Fig1:
Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (HA500523) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: HEK-293 cell lysate Lane 3: MCF-7 cell lysate Lane 4: MDA-MB-468 cell lysate Lane 5: SH-SY5Y cell lysate Lane 6: HUVEC cell lysate Lane 7: Jurkat cell lysate Lane 8: COS-1 cell lysate Lane 9: VERO cell lysate Lane 10: NIH/3T3 cell lysate Lane 11: PC-12 cell lysate Lane 12: Neuro-2a cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500523) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling Cofilin with Rabbit anti-Cofilin antibody (HA500523) at 10μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (HA500523) at 10μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Flow cytometric analysis of Hela cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500523, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Flow cytometric analysis of NIH/3T3 cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500523, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.