KDM6A Rabbit Polyclonal Antibody
cat.: HA500524
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, IP, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: -15℃ to -25℃/1 year (Do not lower than -25℃)
Storage buffer: PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 154 kDa
Isotype: IgG
Immunogen: The specific immunogen used to produce this antibody is proprietary information.
Positive control: HEK-293 (Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  IP
  ELISA

1:2,000-1:10,000
1:200-1:1,000
1:200-1:1,000
1:50-1:200
1:5,000-1:20,000
Uniprot #: SwissProt: O15550 Human | O70546 Mouse
Alternative names: bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) bA386N14.2 bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX DKFZp686A03225 Histone demethylase UTX KABUK2 Kdm6a KDM6A_HUMAN Lysine (K) specific demethylase 6A Lysine K specific demethylase 6A Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome Lysine-specific demethylase 6A MGC141941 Ubiquitously transcribed tetratricopeptide repeat X chromosome Ubiquitously transcribed tetratricopeptide repeat protein X linked Ubiquitously transcribed TPR gene on the X chromosome Ubiquitously transcribed TPR protein on the X chromosome Ubiquitously transcribed X chromosome tetratricopeptide repeat protein Ubiquitously-transcribed TPR protein on the X chromosome Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein UTX
Images
HA500524_1.jpg Fig1: Western blot analysis of KDM6A on different lysates with Rabbit anti-KDM6A antibody (HA500524) at 1/2,000 dilution.

Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

THP-1 is a negative control (PMID: 19330029).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500524, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 154 kDa
Observed band size: 154 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.