| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IP, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | -15℃ to -25℃/1 year (Do not lower than -25℃) |
| Storage buffer: | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 154 kDa |
| Isotype: | IgG |
| Immunogen: | The specific immunogen used to produce this antibody is proprietary information. |
| Positive control: | HEK-293 (Human embryonic kidney cell) cell lysate, NIH/3T3 (Mouse fibroblast) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell IP ELISA |
1:2,000-1:10,000 1:200-1:1,000 1:200-1:1,000 1:50-1:200 1:5,000-1:20,000 |
| Uniprot #: | SwissProt: O15550 Human | O70546 Mouse |
| Alternative names: | bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) bA386N14.2 bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX DKFZp686A03225 Histone demethylase UTX KABUK2 Kdm6a KDM6A_HUMAN Lysine (K) specific demethylase 6A Lysine K specific demethylase 6A Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome Lysine-specific demethylase 6A MGC141941 Ubiquitously transcribed tetratricopeptide repeat X chromosome Ubiquitously transcribed tetratricopeptide repeat protein X linked Ubiquitously transcribed TPR gene on the X chromosome Ubiquitously transcribed TPR protein on the X chromosome Ubiquitously transcribed X chromosome tetratricopeptide repeat protein Ubiquitously-transcribed TPR protein on the X chromosome Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein UTX |
|
Fig1:
Western blot analysis of KDM6A on different lysates with Rabbit anti-KDM6A antibody (HA500524) at 1/2,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 THP-1 is a negative control (PMID: 19330029). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA500524, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 154 kDa Observed band size: 154 kDa |