CXCR1 Rabbit Polyclonal Antibody
cat.: HA500526
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within full length human CXCR1 protein. |
| Positive control: | U87-MG cell lysate, Hela cell lysate, Jurkat cell lysate, HepG2 cell lysate, Hela. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P25024 Human |
| Alternative names: | C C C C CKR 1 C-X-C chemokine receptor type 1 CCCKR1 CD 181 CD128 CD181 CD181 antigen CDw128a Chemokine (C X C motif) receptor 1 Chemokine (C X C) receptor 1 Chemokine receptor 1 CKR 1 CMKAR1 CXC-R1 CXCR 1 CXCR-1 CXCR1 CXCR1_HUMAN High affinity interleukin 8 receptor A High affinity interleukin-8 receptor A IL 8 receptor type 1 IL 8R A IL-8 receptor type 1 IL-8R A IL8 receptor IL8 receptor type 1 IL8R1 IL8RA IL8RBA Interleukin 8 receptor alpha Interleukin 8 receptor type 1 Interleukin 8 receptor type A |
Images
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Fig1:
Western blot analysis of CXCR1 on different lysates with Rabbit anti-CXCR1 antibody (HA500526) at 1/1,000 dilution. Lane 1: U87-MG cell lysate Lane 2: Hela cell lysate Lane 3: Jurkat cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 40 kDa Observed band size: 38 kDa Exposure time: 50 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500526) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling CXCR1 with Rabbit anti-CXCR1 antibody (HA500526) at 10μg/mL dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CXCR1 antibody (HA500526) at 10μg/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.