TATA binding protein TBP Rabbit Polyclonal Antibody
cat.: HA500527
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TBP aa 140-339 / 339. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, HepG2 cell lysate, MCF7 cell lysate, Jurkat cell lysate, C2C12 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, RAW264.7 cell lysate, mouse testis tissue lysate, human bladder carcinoma tissue, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:2,000 |
| Uniprot #: | SwissProt: P20226 Human | P29037 Mouse Entrez Gene: 117526 Rat |
| Alternative names: | GTF2D GTF2D1 HDL4 MGC117320 MGC126054 MGC126055 SCA17 TATA binding factor TATA box factor TATA sequence binding protein TATA sequence-binding protein TATA-binding factor TATA-box binding protein N-terminal domain TATA-box factor TATA-box-binding protein TBP TBP_HUMAN TFIID Transcription initiation factor TFIID TBP subunit |
Images
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Fig1:
Western blot analysis of TATA binding protein TBP on different lysates with Rabbit anti-TATA binding protein TBP antibody (HA500527) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HCT 116 cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: HepG2 cell lysate (10 µg/Lane) Lane 5: MCF7 cell lysate (10 µg/Lane) Lane 6: Jurkat cell lysate (10 µg/Lane) Lane 7: C2C12 cell lysate (10 µg/Lane) Lane 8: NIH/3T3 cell lysate (10 µg/Lane) Lane 9: PC-12 cell lysate (10 µg/Lane) Lane 10: RAW264.7 cell lysate (10 µg/Lane) Lane 11: Mouse testis tissue lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 40/36 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500527) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-TATA binding protein TBP antibody (HA500527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TATA binding protein TBP antibody (HA500527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.