CSNK2A1 Rabbit Polyclonal Antibody
cat.: HA500528
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CSNK2A1 aa 1-150. |
| Positive control: | AGS cell lysate, HepG2 cell lysate, A431 cell lysate, mouse colon tissue lysate, mouse kidney tissue lysate, human thyroid tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P68400 Human | Q60737 Mouse |
| Alternative names: | Casein kinase 2 alpha 1 polypeptide Casein kinase II alpha 1 Casein kinase II alpha 1 subunit Casein kinase II alpha subunit Casein kinase II subunit alpha CK II alpha CK II CK2 alpha CK2 catalytic subunit alpha CK2A1 CKII CKIIalpha CSK21_HUMAN CSNK2A1 |
Images
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Fig1:
Western blot analysis of CSNK2A1 on different lysates with Rabbit anti-CSNK2A1 antibody (HA500528) at 1/1,000 dilution. Lane 1: AGS cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: Mouse colon tissue lysate (40 µg/Lane) Lane 5: Mouse kidney tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 40/37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500528) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-CSNK2A1 antibody (HA500528) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.