EPO-R Rabbit Polyclonal Antibody
cat.: HA500529
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human EPO-R aa 351-400 / 508. |
| Positive control: | K-562 cell lysate, HepG2 cell lysate. |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P19235 Human |
| Alternative names: | EPO R EPO Receptor EPO-R epor EPOR_HUMAN Erythropoietin receptor Erythropoietin receptor precursor MGC138358 |
Images
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Fig1:
Western blot analysis of EPO-R on different lysates with Rabbit anti-EPO-R antibody (HA500529) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 25 µg/Lane. Predicted band size: 55 kDa Observed band size: 56 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500529) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.