MAVS Rabbit Polyclonal Antibody
cat.: HA500530
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAVS aa 1-540 / 540. |
| Positive control: | A549 cell lysate, MCF7 cell lysate, Jurkat cell lysate, HeLa cell lysate, A431 cell lysate, THP-1 cell lysate, HCT 116 cell lysate, HepG2 cell lysate, A431, human pancreas tissue. |
| Subcellular location: | Membrane, Mitochondrion, Mitochondrion outer membrane, Peroxisome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:200 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q7Z434 Human |
| Alternative names: | CARD adapter inducing interferon beta CARD adaptor inducing IFN beta Cardif DKFZp666M015 FLJ27482 FLJ41962 IFN B promoter stimulator 1 Interferon beta promoter stimulator protein 1 Ips 1 IPS-1 Ips1 KIAA1271 MAVS MAVS_HUMAN Mitochondrial anti viral signaling protein Mitochondrial Antiviral Signaling Mitochondrial antiviral signaling protein Mitochondrial antiviral-signaling protein Putative NF kappa B activating protein 031N Putative NF-kappa-B-activating protein 031N Virus induced signaling adapter virus induced signaling adaptor Virus-induced-signaling adapter VISA |
Images
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Fig1:
Western blot analysis of MAVS on different lysates with Rabbit anti-MAVS antibody (HA500530) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: MCF7 cell lysate Lane 3: Jurkat cell lysate Lane 4: HeLa cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: HCT 116 cell lysate Lane 8: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56/75 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500530) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A431 cells labeling MAVS with Rabbit anti-MAVS antibody (HA500530) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MAVS antibody (HA500530) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-MAVS antibody (HA500530) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500530) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Flow cytometric analysis of A431 cells labeling MAVS. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500530, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.