LZTS1 / FEZ1 Rabbit Polyclonal Antibody
cat.: HA500531
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 67 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human LZTS1 aa 201-596 / 596. |
| Positive control: | Jurkat cell lysate, 293T cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, Jurkat, human brain tissue. |
| Subcellular location: | Cytoplasm, Cell membrane, Cell projection, dendritic spine, Postsynaptic density, Synapse. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:100 1:200 |
| Uniprot #: | SwissProt: Q9Y250 Human | P60853 Mouse | Q8CFC9 Rat |
| Alternative names: | F37 F37/esophageal cancer related gene coding leucine zipper motif F37/esophageal cancer-related gene-coding leucine-zipper motif Fez1 Leucine zipper putative tumor suppressor 1 Lzts1 LZTS1_HUMAN Psdzip70 |
Images
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Fig1:
Western blot analysis of LZTS1 / FEZ1 on different lysates with Rabbit anti-LZTS1 / FEZ1 antibody (HA500531) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 67 kDa Observed band size: 67 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500531) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling LZTS1 / FEZ1 with Rabbit anti-LZTS1 / FEZ1 antibody (HA500531) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LZTS1 / FEZ1 antibody (HA500531) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-LZTS1 / FEZ1 antibody (HA500531) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500531) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.