cIAP1/2 Rabbit Polyclonal Antibody
cat.: HA500535
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 70/68 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human cIAP1 aa 201-618 / 618. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, HT-29 cell lysate, THP-1 cell lysate, TF-1 cell lysate, F9 cell lysate, A549 cell lysate, HEK-293 cell lysate, HT-29, HeLa. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q13490 Human | Q13489 Human | Q62210 Mouse | O08863 Mouse |
| Alternative names: | API 1 API1 Apoptosis inhibitor 1 Baculoviral IAP repeat containing 2 Baculoviral IAP repeat containing protein 2 Baculoviral IAP repeat-containing protein 2 BIRC 2 BIRC2 BIRC2_HUMAN C IAP1 C-IAP1 Cellular inhibitor of apoptosis 1 cellular inhibitor of apoptosis protein 1 cIAP 1 cIAP1 HIAP 2 HIAP-2 HIAP2 IAP 2 IAP homolog B IAP-2 IAP2 Inhibitor of apoptosis protein 2 MIHB NFR2 TRAF signalling complex protein RING finger protein 48 RNF 48 RNF48 TNFR2 TRAF signaling complex protein 2 TNFR2-TRAF-signaling complex protein 2 |
Images
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Fig1:
Western blot analysis of cIAP1/2 on different lysates with Rabbit anti-cIAP1/2 antibody (HA500535) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: Jurkat cell lysate Lane 4: HT-29 cell lysate Lane 5: THP-1 cell lysate Lane 6: TF-1 cell lysate Lane 7: F9 cell lysate Lane 8: A549 cell lysate Lane 9: HEK-293 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70/68 kDa Observed band size: 70/68 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500535) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HT-29 cells labeling cIAP1/2 with Rabbit anti-cIAP1/2 antibody (HA500535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-cIAP1/2 antibody (HA500535) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling cIAP1/2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500535, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.