CrkL Rabbit Polyclonal Antibody
cat.: HA500536
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.053ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CrkL aa 1-303 / 303. |
| Positive control: | K-562 cell lysate, 293T cell lysate, MCF7 cell lysate, Jurkat cell lysate, HT-29 cell lysate, THP-1 cell lysate, HeLa cell lysate, A431 cell lysate, HL-60 cell lysate, C6 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, K-562. |
| Subcellular location: | Cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P46109 Human | P47941 Mouse | Q5U2U2 Rat |
| Alternative names: | Crk L Crk like protein Crk-like protein Crkl CRKL_HUMAN Crkol HGNC:2363 Oncogene CrkL V crk avian sarcoma virus CT10 oncogene homolog like v crk sarcoma virus CT10 oncogene homolog (avian) like V crk sarcoma virus CT10 oncogene homolog avian like |
Images
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Fig1:
Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA500536) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: Jurkat cell lysate Lane 5: HT-29 cell lysate Lane 6: THP-1 cell lysate Lane 7: HeLa cell lysate Lane 8: A431 cell lysate Lane 9: HL-60 cell lysate Lane 10: C6 cell lysate Lane 11: Mouse thymus tissue lysate Lane 12: Rat thymus tissue lysate Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 40 µg/Lane. Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500536) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of K-562 cells labeling CrkL with Rabbit anti-CrkL antibody (HA500536) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CrkL antibody (HA500536) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of K-562 cells labeling CrkL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500536, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.