CrkL Rabbit Polyclonal Antibody
cat.: HA500536
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.053ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CrkL aa 1-303 / 303. |
| Positive control: | K-562 cell lysate, 293T cell lysate, Jurkat cell lysate, HT-29 cell lysate, THP-1 cell lysate, A431 cell lysate, HL-60 cell lysate, C6 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate, K-562. |
| Subcellular location: | Cytosol, extrinsic component of postsynaptic membrane, neuromuscular junction, nucleoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: P46109 Human | P47941 Mouse | Q5U2U2 Rat |
| Alternative names: | Crk L Crk like protein Crk-like protein Crkl CRKL_HUMAN Crkol HGNC:2363 Oncogene CrkL V crk avian sarcoma virus CT10 oncogene homolog like v crk sarcoma virus CT10 oncogene homolog (avian) like V crk sarcoma virus CT10 oncogene homolog avian like |
Images
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Fig1:
Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA500536) at 1/2,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: Jurkat cell lysate (20 µg/Lane) Lane 4: HT-29 cell lysate (20 µg/Lane) Lane 5: THP-1 cell lysate (20 µg/Lane) Lane 6: A431 cell lysate (20 µg/Lane) Lane 7: HL-60 cell lysate (low expression) (20 µg/Lane) Lane 8: C6 cell lysate (20 µg/Lane) Lane 9: Mouse thymus tissue lysate (40 µg/Lane) Lane 10: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 34 kDa Observed band size: 37 kDa Exposure time: 40 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500536) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of K-562 cells labeling CrkL with Rabbit anti-CrkL antibody (HA500536) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CrkL antibody (HA500536) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of K-562 cells labeling CrkL. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500536, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.