Homer2 Rabbit Polyclonal Antibody
cat.: HA500538
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 41 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Homer2 aa 284-333 / 354.
Positive control: HepG2 cell lysate, LO2 cell lysate, Huh7 cell lysate, A549 cell lysate.
Subcellular location: Cell membrane, cytoplasm, postsynaptic density, synapse, stereocilium.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9NSB8 Human
Alternative names: Homer protein homolog 2 Homer-2 Cupidin HOMER2
Images
HA500538_1.jpg Fig1: Western blot analysis of Homer2 on different lysates with Rabbit anti-Homer2 antibody (HA500538) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: LO2 cell lysate
Lane 3: Huh7 cell lysate
Lane 4: A549 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 41 kDa
Observed band size: 48 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500538) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.