ALDH1A3 Rabbit Polyclonal Antibody
cat.: HA500541
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ALDH1A3 aa 1-512 / 512. |
| Positive control: | HepG2 cell lysate, SW1990 cell lysate, U-87 MG cell lysate, A431 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P47895 Human | Q9JHW9 Mouse | Q8K4D8 Rat |
| Alternative names: | Acetaldehyde dehydrogenase 6 AL1A3_HUMAN Aldehyde dehydrogenase 1A3 Aldehyde dehydrogenase 6 Aldehyde dehydrogenase family 1 member A3 Aldh1a3 ALDH1A6 ALDH6 RALDH 3 Retinaldehyde dehydrogenase 3 V1 |
Images
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Fig1:
Western blot analysis of ALDH1A3 on different lysates with Rabbit anti-ALDH1A3 antibody (HA500541) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: SW1990 cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: A431 cell lysate (20 µg/Lane) Lane 5: Human kidney tissue lysate (40 µg/Lane) Lane 6: Human liver tissue lysate (40 µg/Lane) Lane 7: Mouse kidney tissue lysate (40 µg/Lane) Lane 8: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500541) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.