HIP2 / LIG Rabbit Polyclonal Antibody
cat.: HA500542
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HIP2 aa 1-200 / 200. |
| Positive control: | 293T cell lysate, HeLa cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P61086 Human | P61087 Mouse Entrez Gene: 289623 Rat |
| Alternative names: | E2 25K E2(25K) HIP-2 Huntingtin interacting protein 2 Huntingtin-interacting protein 2 HYPG LIG UBC1 UBE2K UBE2K_HUMAN Ubiquitin carrier protein Ubiquitin conjugating enzyme E2 25 kDa ubiquitin conjugating enzyme E2K ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) Ubiquitin protein ligase Ubiquitin-conjugating enzyme E2 K Ubiquitin-conjugating enzyme E2(25K) Ubiquitin-conjugating enzyme E2-25 kDa Ubiquitin-conjugating enzyme E2-25K Ubiquitin-protein ligase |
Images
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Fig1:
Western blot analysis of HIP2 / LIG on different lysates with Rabbit anti-HIP2 / LIG antibody (HA500542) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 22 kDa Observed band size: 25 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500542) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.