MNK2 Rabbit Polyclonal Antibody
cat.: HA500543
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MNK2 aa 266-465 / 465. |
| Positive control: | Jurkat cell lysate, HeLa cell lysate, SK-OV-3 cell lysate, HepG2 cell lysate, rat ovary tissue lysate, mouse testis tissue, rat skeletal muscle tissue. |
| Subcellular location: | Nucleus, PML body; Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q9HBH9 Human | Q8CDB0 Mouse | Q5U2N4 Rat |
| Alternative names: | G protein coupled receptor kinase 7 GPRK7 MAP kinase interacting serine threonine kinase 2 MAP kinase interacting serine threonine kinase MAP kinase interacting serine threonine protein kinase 2 MAP kinase signal integrating kinase 2 MAP kinase signal-integrating kinase 2 MAP kinase-interacting serine/threonine-protein kinase 2 MAPK interacting kinase 2 MAPK signal integrating kinase 2 Mitogen activated protein kinase interacting serine threonine kinase 2 Mknk2 MKNK2_HUMAN Mnk2 Putative map kinase interacting kinase |
Images
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Fig1:
Western blot analysis of MNK2 on different lysates with Rabbit anti-MNK2 antibody (HA500543) at 1/1,000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: SK-OV-3 cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: Rat ovary tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500543) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-MNK2 antibody (HA500543) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500543) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MNK2 antibody (HA500543) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500543) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.