NSUN5 Rabbit Polyclonal Antibody
cat.: HA500544
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human NSUN5 aa 51-100 / 429. |
| Positive control: | HeLa cell lysate, A549 cell lysate, Caco-2 cell lysate, K-562 cell lysate, A431 cell lysate, L-929 cell lysate, MEF cell lysate, PC-12 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, human skeletal muscle tissue, human placenta tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Nucleus, nucleolus. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: Q96P11 Human | Q8K4F6 Mouse Entrez Gene: 288595 Rat |
| Alternative names: | FLJ10267 MGC986 NOL1 NOL1-related protein NOL1/NOP2/Sun domain family member 5 NOL1R NOP2/Sun domain family, member 5 NOP2/Sun domain family, member 5A NSUN5 NSUN5_HUMAN p120 Putative methyltransferase NSUN5 WBSCR20 WBSCR20A Williams Beuren syndrome chromosome region 20A Williams-Beuren syndrome chromosomal region 20A protein Williams-Beuren syndrome critical region protein 20 copy A |
Images
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Fig1:
Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: Caco-2 cell lysate Lane 4: K-562 cell lysate Lane 5: A431 cell lysate Lane 6: L-929 cell lysate Lane 7: MEF cell lysate Lane 8: PC-12 cell lysate Lane 9: Human kidney tissue lysate Lane 10: Mouse kidney tissue lysate Lane 11: Mouse heart tissue lysate Lane 12: Mouse spleen tissue lysate Lane 13: Rat kidney tissue lysate Lane 14: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500544) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NSUN5 antibody (HA500544) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500544) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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