PORCN Rabbit Polyclonal Antibody
cat.: HA500546
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Peptide affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Positive control: | K-562 cell lysate, HepG2 cell lysate, SiHa cell lysate, 293T cell lysate, SH-SY5Y cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat kidney tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9H237 Human | Q9JJJ7 Mouse Entrez Gene: 317368 Rat |
| Alternative names: | DHOF FODH MG61 MGC29687 por PORC PORCN PORCN_HUMAN PPN Probable protein-cysteine N-palmitoyltransferase porcupine Protein MG61 |
Images
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Fig1:
Western blot analysis of PORCN on different lysates with Rabbit anti-PORCN antibody (HA500546) at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: SiHa cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Lane 6: Neuro-2a cell lysate (20 µg/Lane) Lane 7: C6 cell lysate (20 µg/Lane) Lane 8: Mouse kidney tissue lysate (40 µg/Lane) Lane 9: Mouse brain tissue lysate (40 µg/Lane) Lane 10: Rat brain tissue lysate (40 µg/Lane) Lane 11: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 37 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500546) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.