PELO Rabbit Polyclonal Antibody
cat.: HA500548
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.4ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PELO aa 1-385. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, RAW264.7 cell lysate, Neuro-2a cell lysate, C6 cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9BRX2 Human | Q80X73 Mouse | Q5XIP1 Rat |
| Alternative names: | CGI 17 CGI17 pelo PELO_HUMAN pelota (Drosophila) homolog pelota homolog PRO1770 Protein pelota homolog |
Images
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Fig1:
Western blot analysis of PELO on different lysates with Rabbit anti-PELO antibody (HA500548) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: RAW264.7 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500548) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.