ANGPTL6 Rabbit Polyclonal Antibody
cat.: HA500551
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ANGPTL6 aa 105-140/470 |
| Positive control: | Neuro-2a cell lysate, 3T3-L1 cell lysate, J774A.1 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Neuro-2a, J774A.1, mouse liver tissue. |
| Subcellular location: | Secreted |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:2,000 1:200-1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q8NI99 Human | Q8R0Z6 Mouse | A6JNL9 Rat |
| Alternative names: | AGF Angiopoietin like 6 Angiopoietin like Protein 6 Angiopoietin related Growth Factor Angiopoietin related protein 5 Angiopoietin-like protein 6 Angiopoietin-related growth factor Angiopoietin-related protein 5 Angiopoietin-related protein 6 ANGL6_HUMAN Angptl6 ARP5 |
Images
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Fig1:
Western blot analysis of ANGPTL6 on different lysates with Rabbit anti-ANGPTL6 antibody (HA500551) at 1/2,000 dilution. Lane 1: Neuro-2a cell lysate (20 µg/Lane) Lane 2: 3T3-L1 cell lysate (20 µg/Lane) Lane 3: J774A.1 cell lysate (20 µg/Lane) Lane 4: mouse brain tissue lysate (40 µg/Lane) Lane 5: rat brain tissue lysate (40 µg/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500551) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Neuro-2a cells labeling ANGPTL6 with Rabbit anti-ANGPTL6 antibody (HA500551) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ANGPTL6 antibody (HA500551) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of J774A.1 cells labeling ANGPTL6 with Rabbit anti-ANGPTL6 antibody (HA500551) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ANGPTL6 antibody (HA500551) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ANGPTL6 antibody (HA500551) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500551) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of J774A.1 cells labeling ANGPTL6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500551, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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