Actin Rabbit Polyclonal Antibody
cat.: HA500552
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Arabidopsis thaliana |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Positive control: | Oryza sativa lysate, Cotton lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P53496 ARATH |
| Alternative names: | Actin-11 EC:3.6.4.- ACT11 T21B14.7 T21B14_108 T23B7.5 At3g12110 |
Images
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Fig1:
Western blot analysis of Actin on different lysates with Rabbit anti-Actin antibody (HA500552) at 1/2,000 dilution. Lane 1: Oryza sativa lysate Lane 2: Cotton lysate Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: Lane 1: 25 seconds; Lane 2: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500552) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.