TMEM50A Rabbit Polyclonal Antibody
cat.: HA500556
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse TMEM50A aa 11-60. |
| Positive control: | Mouse colon tissue lysate, Mouse lung tissue lysate, Mouse kidney tissue lysate, Mouse spleen tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9CXL1 Mouse |
| Alternative names: | Small membrane protein 1 TM50A_HUMAN Tmem50a Transmembrane protein 50A Transmembrane protein 50A Small membrane protein 1 Tmem50a Smp1 |
Images
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Fig1:
Western blot analysis of TMEM50A on different lysates with Rabbit anti-TMEM50A antibody (HA500556) at 1/1,000 dilution. Lane 1: Mouse colon tissue lysate Lane 2: Mouse lung tissue lysate (no heat) Lane 3: Mouse kidney tissue lysate (no heat) Lane 4: Mouse spleen tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 30 µg/Lane. Predicted band size: 17 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500556) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.