Repulsive guidance molecule A Rabbit Polyclonal Antibody
cat.: HA500559
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RGMA aa 31-440. |
| Positive control: | SH-SY5Y cell lysate, HeLa cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q96B86 Human |
| Alternative names: | Repulsive guidance molecule A RGM RGM domain family member A RGMA RGMA_HUMAN |
Images
|
Fig1:
Western blot analysis of Repulsive guidance molecule A on different lysates with Rabbit anti-Repulsive guidance molecule A antibody (HA500559) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500559) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.