GM2A Rabbit Polyclonal Antibody
cat.: HA500560
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 21 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human GM2A aa 1-193. |
| Positive control: | SK-Br-3 cell lysate, HeLa cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, rat kidney tissue. |
| Subcellular location: | Lysosome. |
| Recommended Dilutions:
WB IHC-P IP |
1:5,000 1:200-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P17900 Human | Q60648 Mouse Entrez Gene: 282838 Rat |
| Alternative names: | Cerebroside sulfate activator protein ganglioside GM2 activator Ganglioside GM2 activator isoform short Ganglioside GM2 activator precursor GM2 activator GM2 AP GM2 ganglioside activator GM2 ganglioside activator protein GM2-AP GM2A GM2AP OTTHUMP00000160619 SAP 3 SAP-3 SAP3 SAP3_HUMAN Shingolipid activator protein 3 Sphingolipid activator protein 3 |
Images
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Fig1:
Western blot analysis of GM2A on different lysates with Rabbit anti-GM2A antibody (HA500560) at 1/5,000 dilution. Lane 1: SK-Br-3 cell lysate (20 µg/Lane) Lane 2: MDA-MB-231 cell lysate (low expression) (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (40 µg/Lane) Lane 5: Mouse skeletal muscle tissue lysate (negative) (40 µg/Lane) Lane 6: Rat kidney tissue lysate (40 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (negative) (40 µg/Lane) Predicted band size: 21 kDa Observed band size: 18 kDa Exposure time: 1 minute 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500560) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GM2A antibody (HA500560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue (negative) with Rabbit anti-GM2A antibody (HA500560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-GM2A antibody (HA500560) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue (negative) with Rabbit anti-GM2A antibody (HA500560) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500560) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
GM2A was immunoprecipitated from 0.2 mg SK-Br-3 cell lysate with HA500560 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA500560 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: SK-Br-3 cell lysate (input) Lane 2: HA500560 IP in SK-Br-3 cell lysate Lane 3: Rabbit IgG instead of HA500560 in SK-Br-3 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 4 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.