DNA helicase HEL308 Rabbit Polyclonal Antibody
cat.: HA500562
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 124 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human DNA helicase HEL308 aa 400-450. |
| Positive control: | U-2 OS cell lysate, MCF7 cell lysate, HeLa cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB |
1:6,000 |
| Uniprot #: | SwissProt: Q8TDG4 Human |
| Alternative names: | HEL308 protein Helicase POLQ-like Helicase, POLQ like HELQ HELQ_HUMAN Mus308 like helicase Mus308-like helicase POLQ like helicase POLQ-like helicase |
Images
|
Fig1:
Western blot analysis of DNA helicase HEL308 on different lysates with Rabbit anti-DNA helicase HEL308 antibody (HA500562) at 1/6,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: Mouse testis tissue lysate Lane 5: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 124 kDa Observed band size: 124 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500562) at 1/6,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.