Ferredoxin-2 Rabbit Polyclonal Antibody
cat.: HA500563
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2.5ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 19 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Mouse Ferredoxin-2 aa 125-174.
Positive control: U-87 MG cell lysate, Mouse testis tissue lysate, Mouse colon tissue lysate.
Subcellular location: Mitochondrion, Mitochondrion matrix.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q6P4F2 Human | Q9CPW2 Mouse
Alternative names: Ferredoxin-2, mitochondrial Adrenodoxin-like protein Ferredoxin-1-like protein FDX2 FDX1L
Images
HA500563_1.jpg Fig1: Western blot analysis of Ferredoxin-2 on different lysates with Rabbit anti-Ferredoxin-2 antibody (HA500563) at 1/5,000 dilution.

Lane 1: U-87 MG cell lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Mouse colon tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 19 kDa
Observed band size: 15 kDa

Exposure time: 1 minute 34 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500563) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.