ELL3 Rabbit Polyclonal Antibody
cat.: HA500564
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse ELL3 aa 100-395. |
| Positive control: | Mouse testis tissue lysate, Mouse spleen tissue lysate, Rat testis tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:5,000-1:20,000 |
| Uniprot #: | SwissProt: Q80VR2 Mouse | Q5XFX8 Rat |
| Alternative names: | Elongation Factor RNA Polymerase II-Like 3 RNA polymerase II elongation factor ELL3 ELL3 |
Images
|
Fig1:
Western blot analysis of ELL3 on different lysates with Rabbit anti-ELL3 antibody (HA500564) at 1/5,000 dilution. Lane 1: Mouse testis tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat testis tissue lysate Lane 4: Rat spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 45 kDa Observed band size: 60 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500564) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.