Alx1 Rabbit Polyclonal Antibody
cat.: HA500565
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Alx1 aa 1-326. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, Mouse articular cartilage tissue lysate, Rat brain tissue lysate, Rat embryo tissue lysate, human trachea tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:500 |
| Uniprot #: | SwissProt: Q15699 Human | Q8C8B0 Mouse | Q63087 Rat |
| Alternative names: | ALX homeobox 1 ALX homeobox protein 1 ALX1 ALX1_HUMAN CART 1 CART-1 CART1 Cartilage homeoprotein 1 Cartilage paired class homeoprotein 1 FND3 |
Images
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Fig1:
Western blot analysis of Alx1 on different lysates with Rabbit anti-Alx1 antibody (HA500565) at 1/5,000 dilution. Lane 1: MCF7 cell lysate (30 µg/Lane) Lane 2: HepG2 cell lysate (30 µg/Lane) Lane 3: Mouse articular cartilage tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Lane 5: Rat embryo tissue lysate (20 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500565) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-Alx1 antibody (HA500565) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500565) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-Alx1 antibody (HA500565) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500565) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.