POLR2A Rabbit Polyclonal Antibody
cat.: HA500566
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 217 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human POLR2A aa 1,601-1,650. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P24928 Human | P08775 Mouse Entrez Gene: 363633 Rat |
| Alternative names: | DNA-directed RNA polymerase II subunit RPB1 RNA polymerase II subunit B1 3'-5' exoribonuclease DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit RNA-directed RNA polymerase II subunit RPB1 POLR2A POLR2 |
Images
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Fig1:
Western blot analysis of POLR2A on different lysates with Rabbit anti-POLR2A antibody (HA500566) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: 293T cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 217 kDa Observed band size: 250 kDa Exposure time: 1 minute; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500566) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.