FCN1 / M-Ficolin Rabbit Polyclonal Antibody
cat.: HA500567
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human FCN1 aa 70-120. |
| Positive control: | HeLa cell lysate, NIH/3T3 cell lysate, U-937. |
| Subcellular location: | Secreted, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:2,000 1:50 |
| Uniprot #: | SwissProt: O00602 Human | O70165 Mouse |
| Alternative names: | Collagen/fibrinogen domain containing protein 1 Collagen/fibrinogen domain-containing protein 1 Fcn1 FCN1_HUMAN FCNM ficolin (collagen/fibrinogen domain containing) 1 Ficolin 1 Ficolin A Ficolin alpha Ficolin-1 Ficolin-A Ficolin-alpha M ficolin M-ficolin |
Images
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Fig1:
Western blot analysis of FCN1 / M-Ficolin on different lysates with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa cell lysate treated with deglycosylation Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 cell lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35/70 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of U-937 cells labeling FCN1 / M-Ficolin with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FCN1 / M-Ficolin antibody (HA500567) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.