| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPARA aa 151-468. |
| Positive control: | Mouse liver tissue lysate, Mouse skeletal muscle tissue lysate, Rat liver tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P23204 Mouse | P35396 Mouse | P37238 Mouse | P37230 Rat | Q62879 Rat | O88275 Rat |
| Alternative names: | hPPAR MGC2237 MGC2452 NR1C1 Nuclear receptor subfamily 1 group C member 1 OTTHUMP00000197740 OTTHUMP00000197741 Peroxisome proliferative activated receptor alpha Peroxisome proliferator activated receptor alpha Peroxisome proliferator-activated receptor alpha PPAR PPAR-alpha ppara PPARA_HUMAN PPARalpha FAAR MGC3931 NR1C2 NUC1 NUCI NUCII Nuclear hormone receptor 1 Nuclear receptor subfamily 1 group C member 2 Peroxisome proliferative activated receptor delta Peroxisome proliferator-activated receptor beta (PPAR-beta) Peroxisome proliferator-activated receptor beta Peroxisome proliferator-activated receptor delta PPAR beta PPAR-beta PPAR-delta PPAR-ß PPARB ppard PPARD_HUMAN CIMT1 GLM1 NR1C3 Nuclear receptor subfamily 1 group C member 3 OTTHUMP00000185032 OTTHUMP00000185036 Peroxisome proliferator activated nuclear receptor gamma variant 1 Peroxisome proliferator activated receptor gamma 1 Peroxisome Proli...... |
|
Fig1:
Western blot analysis of PPAR on different lysates with Rabbit anti-PPAR antibody (HA500569) at 1/5,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Rat liver tissue lysate Lane 4: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 42/52 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500569) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |