Nrf3 Rabbit Polyclonal Antibody
cat.: HA500570
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse Nrf3 aa 1-100. |
| Positive control: | HeLa cell lysate, 3T3-L1 cell lysate, Mouse placenta tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q9Y4A8 Human | Q9WTM4 Mouse |
| Alternative names: | Nuclear factor erythroid 2-related factor 3 NF-E2-related factor 3; NFE2-related factor 3 Nuclear factor, erythroid derived 2, like 3 Nfe2l3 Nrf3 |
Images
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Fig1:
Western blot analysis of Nrf3 on different lysates with Rabbit anti-Nrf3 antibody (HA500570) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: 3T3-L1 cell lysate Lane 3: Mouse placenta tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 73 kDa Observed band size: 73 kDa Exposure time: Lane 1: 10 seconds; Lane 2: 28 seconds; Lane 3: 46 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500570) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.