HES6 Rabbit Polyclonal Antibody
cat.: HA500577
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse HES6 aa 1-50. |
| Positive control: | U-937 cell lysate, HeLa cell lysate, 293T cell lysate, U-2 OS cell lysate, PC-12 cell lysate, PC-12. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:10,000 1:400 |
| Uniprot #: | SwissProt: Q96HZ4 Human Entrez Gene: 316626 Rat |
| Alternative names: | bHLHb41 bHLHc23 C HAIRY1 Class B basic helix-loop-helix protein 41 Hairy and enhancer of split 6 Hairy/enhancer of split 6, Drosophila, homolog of, 6 HES 6 hes family bHLH transcription factor 6 Transcription cofactor HES6 |
Images
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Fig1:
Western blot analysis of HES6 on different lysates with Rabbit anti-HES6 antibody (HA500577) at 1/10,000 dilution. Lane 1: U-937 cell lysate Lane 2: HeLa cell lysate Lane 3: 293T cell lysate Lane 4: U-2 OS cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 24 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500577) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling HES6 with Rabbit anti-HES6 antibody (HA500577) at 1/400 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HES6 antibody (HA500577) at 1/400 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.