DCI / Eci1 Rabbit Polyclonal Antibody
cat.: HA500581
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse DCI / Eci1 aa 1-289. |
| Positive control: | RAW264.7 cell lysate, NIH/3T3 cell lysate, Rat colon tissue lysate, NIH/3T3. |
| Subcellular location: | Mitochondrion matrix. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:250 1μg/mL |
| Uniprot #: | SwissProt: P42125 Mouse | P23965 Rat |
| Alternative names: | 2-trans-enoyl-CoA isomerase 3 3,2 trans enoyl Coenzyme A isomerase 3,2 trans-enoyl-CoA isomerase 3,2-trans-enoyl-CoA isomerase, mitochondrial Acetylene allene isomerase D2-enoyl-CoA isomerase D3 D3,D2 enoyl CoA isomerase DCI DCI protein delta(2)-enoyl-CoA isomerase Delta(3) Delta(3),Delta(2) enoyl CoA isomerase Delta3, delta2 enoyl CoA isomerase Dodecenoyl CoA delta Isomerase Dodecenoyl CoA isomerase Dodecenoyl Coenzyme A delta isomerase Dodecenoyl Coenzyme A delta isomerase (3,2 trans enoyl Coenzyme A isomerase) Dodecenoyl Coenzyme A delta isomerase 3,2 trans enoyl Coenzyme A isomerase, isoform CRA a Dodecenoyl-CoA isomerase ECI1 ECI1_HUMAN Enoyl-CoA delta isomerase 1 enoyl-CoA delta isomerase 1, mitochondrial mitochondrial |
Images
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Fig1:
Western blot analysis of DCI / Eci1 on different lysates with Rabbit anti-DCI / Eci1 antibody (HA500581) at 1/5,000 dilution. Lane 1: RAW264.7 cell lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (20 µg/Lane) Lane 3: Rat colon tissue lysate (30 µg/Lane) Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500581) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling DCI / Eci1 with Rabbit anti-DCI / Eci1 antibody (HA500581) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DCI / Eci1 antibody (HA500581) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of NIH/3T3 cells labeling DCI / Eci1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500581, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.