| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human p38 delta / MAPK13 aa 251-300. |
| Positive control: | A431 cell lysate, Caco-2 cell lysate. |
| Subcellular location: | Cytoplasm, cytosol, nucleus. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: O15264 Human |
| Alternative names: | Mitogen-activated protein kinase 13 EC:2.7.11.24 MAP kinase 13 MAPK 13 Mitogen-activated protein kinase p38 delta MAP kinase p38 delta Stress-activated protein kinase 4 MAPK13 PRKM13 SAPK4 |
|
Fig1:
Western blot analysis of p38 delta / MAPK13 on different lysates with Rabbit anti-p38 delta / MAPK13 antibody (HA500582) at 1/5,000 dilution. Lane 1: A431 cell lysate Lane 2: Caco-2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500582) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |