DCBLD2 / ESDN Rabbit Polyclonal Antibody
cat.: HA500585
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG
Immunogen: Recombinant protein within human DCBLD2 / ESDN aa 529-775.
Positive control: U-2 OS cell lysates.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
1:10,000
Uniprot #: SwissProt: Q96PD2 Human
Alternative names: 1700055P21Rik AW146002 CLCP1 coagulation factor V/VIII-homology domains protein 1 CUB and LCCL domain-containing protein 2 CUB CUB, LCCL and coagulation factor V/VIII-homology domains protein 1 DCBD2_HUMAN DCBLD2 Discoidin discoidin CUB and LCCL domain containing 2 Discoidin, CUB and LCCL domain-containing protein 2 Endothelial and smooth muscle cell-derived neuropilin-like protein ESDN LCCL and coagulation factor V/VIII-homology domains protein 1
Images
HA500585_1.jpg Fig1: Western blot analysis of DCBLD2 / ESDN on U-2 OS cell lysate with Rabbit anti-DCBLD2 / ESDN antibody (HA500585) at 1/10,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 85 kDa
Observed band size: 110 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500585) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.