OTULIN Rabbit Polyclonal Antibody
cat.: HA500587
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human OTULIN aa 1-352. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, 786-0 cell lysate, NIH/3T3 cell lysate, COS-1 cell lysate, Mouse brain tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q96BN8 Human | Q3UCV8 Mouse Entrez Gene: 100362554 Rat |
| Alternative names: | AIPDS Deubiquitinating enzyme otulin F105B_HUMAN Fam105b Family with sequence similarity 105, member B FLJ34884 GUM OTU deubiquitinase with linear linkage specificity OTULIN Protein FAM105B Ubiquitin thioesterase Gumby |
Images
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Fig1:
Western blot analysis of OTULIN on different lysates with Rabbit anti-OTULIN antibody (HA500587) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: 786-0 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: COS-1 cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500587) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.