beta COP Rabbit Polyclonal Antibody
cat.: HA500588
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 107 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human beta COP aa 571-620. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, COPI-coated vesicle membrane, Cell membrane, Endoplasmic reticulum-Golgi intermediate compartment. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: P53618 Human | Q9JIF7 Mouse | P23514 Rat |
| Alternative names: | Beta-coat protein Beta-COP betacop Coatomer beta subunit Coatomer protein complex subunit beta 1 Coatomer protein complex subunit beta Coatomer subunit beta COPB COPB_HUMAN Copb1 DKFZp761K102 FLJ10341 |
Images
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Fig1:
Western blot analysis of beta COP on different lysates with Rabbit anti-beta COP antibody (HA500588) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HCT 116 cell lysate Lane 3: 293T cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 107 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500588) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.