AFF4 Rabbit Polyclonal Antibody
cat.: HA500590
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 0.25ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human AFF4 aa 51-250. |
| Positive control: | MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, SP2/0 cell lysate, Rat pancreas tissue lysate, HeLa. |
| Subcellular location: | Nucleus, Chromosome. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:250 1:1,000 |
| Uniprot #: | SwissProt: Q9UHB7 Human | Q9ESC8 Mouse Entrez Gene: 303132 Rat |
| Alternative names: | AF4/FMR2 family member 4 AF5Q31 Alf4 ALL1 fused gene from chromosome 5q31 CHOPS HSPC092 Laf4 Lymphoid nuclear protein related to AF4 like Major CDK9 elongation factor associated protein MCEF MGC107469 RP23-226J16.7 |
Images
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Fig1:
Western blot analysis of AFF4 on different lysates with Rabbit anti-AFF4 antibody (HA500590) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: SP2/0 cell lysate Lane 5: Rat pancreas tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 127 kDa Observed band size: 150 kDa Exposure time: Lane 1-4: 1 minute 12 seconds; Lane 5: 9 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500590) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling AFF4 with Rabbit anti-AFF4 antibody (HA500590) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AFF4 antibody (HA500590) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling AFF4. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.