COL4A3 Rabbit Polyclonal Antibody
cat.: HA500591
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 162 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse COL4A3 aa 141-190. |
| Positive control: | PC-12 cell lysates, PC-12. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:500 |
| Uniprot #: | Entrez Gene: 363265 Rat |
| Alternative names: | Alpha 3 type IV collagen Alpha3 type IV collagen COL4A 3 Col4a3 Collagen alpha 3(IV) chain Collagen IV alpha 3 polypeptide Collagen type IV alpha 3 (Goodpasture antigen) Collagen type IV alpha 3 Collagen type IV alpha 3 chain Goodpasture antigen OTTHUMP00000195044 Tumstatin |
Images
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Fig1:
Western blot analysis of COL4A3 on PC-12 cell lysates with Rabbit anti-COL4A3 antibody (HA500591) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 162 kDa Observed band size: 200 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500591) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling COL4A3 with Rabbit anti-COL4A3 antibody (HA500591) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COL4A3 antibody (HA500591) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.