WWOX Rabbit Polyclonal Antibody
cat.: HA500592
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human WWOX aa 1-414. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Rat brain tissue lysate, HepG2, NIH/3T3. |
| Subcellular location: | Cytoplasm, Nucleus, Mitochondrion, Golgi apparatus, Lysosome. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:500 1:2,000 |
| Uniprot #: | SwissProt: Q9NZC7 Human | Q91WL8 Mouse Entrez Gene: 292041 Rat |
| Alternative names: | 5330426P09Rik 9030416C10Rik Aberrant WW domain-containing oxidoreductase D16S432E EC 1.1.1.- EIEE28 FOR FRA16D Fragile site FRA16D oxidoreductase Fragile site FRA16D Oxireductase HHCMA56 MGC55975 PRO0128 Putative oxidoreductase SCAR12 SDR41C1 Short chain dehydrogenase/reductase family 41C, member 1 WOX1 WW domain containing oxidoreductase WW domain-containing oxidoreductase WW domain-containing protein WWOX wwox WWOX_HUMAN zgc:55975 |
Images
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Fig1:
Western blot analysis of WWOX on different lysates with Rabbit anti-WWOX antibody (HA500592) at 1/5,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: COS-1 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500592) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling WWOX with Rabbit anti-WWOX antibody (HA500592) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WWOX antibody (HA500592) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling WWOX with Rabbit anti-WWOX antibody (HA500592) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WWOX antibody (HA500592) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of HepG2 cells labeling WWOX. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500592, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig5:
Flow cytometric analysis of NIH/3T3 cells labeling WWOX. Cells were fixed and permeabilized. Then stained with the primary antibody (HA500592, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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