TMEM184A Rabbit Polyclonal Antibody
cat.: HA500595
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human TMEM184A aa 191-240.
Positive control: HT-29 cell lysate, AGS cell lysate, Mouse kidney tissue lysate.
Subcellular location: Cell membrane, Cytoplasm, perinuclear region, Cytoplasmic vesicle membrane, Early endosome membrane, Endosome, Cytoplasmic vesicle, secretory vesicle membrane.
Recommended Dilutions:
  WB

1:20,000
Uniprot #: SwissProt: Q6ZMB5 Human | Q3UFJ6 Mouse
Alternative names: Transmembrane protein 184A TMEM184A
Images
HA500595_1.jpg Fig1: Western blot analysis of TMEM184A on different lysates with Rabbit anti-TMEM184A antibody (HA500595) at 1/20,000 dilution.

Lane 1: HT-29 cell lysate
Lane 2: AGS cell lysate
Lane 3: Mouse kidney tissue lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500595) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.