| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TMEM184A aa 191-240. |
| Positive control: | HT-29 cell lysate, AGS cell lysate, Mouse kidney tissue lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, perinuclear region, Cytoplasmic vesicle membrane, Early endosome membrane, Endosome, Cytoplasmic vesicle, secretory vesicle membrane. |
| Recommended Dilutions:
WB |
1:20,000 |
| Uniprot #: | SwissProt: Q6ZMB5 Human | Q3UFJ6 Mouse |
| Alternative names: | Transmembrane protein 184A TMEM184A |
|
Fig1:
Western blot analysis of TMEM184A on different lysates with Rabbit anti-TMEM184A antibody (HA500595) at 1/20,000 dilution. Lane 1: HT-29 cell lysate Lane 2: AGS cell lysate Lane 3: Mouse kidney tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500595) at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |