| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TWSG1 / TSG aa 151-200. |
| Positive control: | A549 cell lysate, SK-OV-3 cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9GZX9 Human |
| Alternative names: | Twisted gastrulation protein homolog 1 TWSG1 TSG PSEC0250 |
|
Fig1:
Western blot analysis of TWSG1 / TSG on different lysates with Rabbit anti-TWSG1 / TSG antibody (HA500597) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: SK-OV-3 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 25 kDa Observed band size: 22 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500597) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |