TWSG1 / TSG Rabbit Polyclonal Antibody
cat.: HA500597
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 25 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human TWSG1 / TSG aa 151-200.
Positive control: A549 cell lysate, SK-OV-3 cell lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q9GZX9 Human
Alternative names: Twisted gastrulation protein homolog 1 TWSG1 TSG PSEC0250
Images
HA500597_1.jpg Fig1: Western blot analysis of TWSG1 / TSG on different lysates with Rabbit anti-TWSG1 / TSG antibody (HA500597) at 1/5,000 dilution.

Lane 1: A549 cell lysate
Lane 2: SK-OV-3 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 25 kDa
Observed band size: 22 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500597) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.