ITM2A Rabbit Polyclonal Antibody
cat.: HA500598
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1.4ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse ITM2A aa 151-263. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, HL-60 cell lysate, A549. |
| Subcellular location: | Membrane; Intracellular. |
| Recommended Dilutions:
WB IF-Cell |
1:10,000 1:5,000 |
| Uniprot #: | SwissProt: O43736 Human |
| Alternative names: | BRICD2A BRICHOS domain containing 2A E25 protein E25A Integral membrane protein 2A ITM2A ITM2A_HUMAN Protein E25 |
Images
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Fig1:
Western blot analysis of ITM2A on different lysates with Rabbit anti-ITM2A antibody (HA500598) at 1/10,000 dilution. Lane 1: A549 cell lysate Lane 2: HepG2 cell lysate Lane 3: HL-60 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 30 kDa Observed band size: 90 kDa Exposure time: 11 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500598) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of A549 cells labeling ITM2A with Rabbit anti-ITM2A antibody (HA500598) at 1/5,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ITM2A antibody (HA500598) at 1/5,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.