SP140 Rabbit Polyclonal Antibody
cat.: HA500602
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse SP140 aa 201-250. |
| Positive control: | Mouse testis tissue lysates. |
| Subcellular location: | Cytoplasm, nucleolus, nucleus. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q6NSQ5 Mouse |
| Alternative names: | Sp140 nuclear body protein Sp140 |
Images
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Fig1:
Western blot analysis of SP140 on mouse testis tissue lysates with Rabbit anti-SP140 antibody (HA500602) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 95 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500602) at 1/2,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.