| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CHAC1 aa 31-80. |
| Positive control: | Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Golgi apparatus, trans-Golgi network. |
| Recommended Dilutions:
WB |
1:2,000-1:10,000 |
| Uniprot #: | SwissProt: Q8R3J5 Mouse | B3STU3 Rat |
| Alternative names: | Blocks Notch protein Botch Cation transport regulator like protein 1 Cation transport regulator-like protein 1 ChaC ChaC cation transport regulator homolog 1 ChaC cation transport regulator like 1 chac1 CHAC1_HUMAN Gamma GCT acting on glutathione homolog 1 Gamma-GCG 1 Glutathione-specific gamma-glutamylcyclotransferase 1 |
|
Fig1:
Western blot analysis of CHAC1 on different lysates with Rabbit anti-CHAC1 antibody (HA500604) at 1/10,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500604) at 1/10,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |