Sorbitol dehydrogenase Rabbit Polyclonal Antibody
cat.: HA500609
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Sorbitol dehydrogenase aa 1-50. |
| Positive control: | HeLa cell lysate, HCT 116 cell lysate, SW620 cell lysate, BxPC-3 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat liver tissue lysate. |
| Subcellular location: | Mitochondrion membrane, Cell projection, cilium, flagellum. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q00796 Human | Q64442 Mouse | P27867 Rat |
| Alternative names: | Sorbitol dehydrogenase EC:1.1.1. SDH (R,R)-butanediol dehydrogenase L-iditol 2-dehydrogenase Polyol dehydrogenase Ribitol dehydrogenase (RDH) Xylitol dehydrogenase (XDH) SORD |
Images
|
Fig1:
Western blot analysis of Sorbitol dehydrogenase on different lysates with Rabbit anti-Sorbitol dehydrogenase antibody (HA500609) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HCT 116 cell lysate (20 µg/Lane) Lane 3: SW620 cell lysate (20 µg/Lane) Lane 4: BxPC-3 cell lysate (20 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Mouse kidney tissue lysate (40 µg/Lane) Lane 7: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 40 kDa Exposure time: 5 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500609) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.