| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SLC52A2 aa 201-250. |
| Positive control: | A549 cell lysate, HeLa cell lysate, MCF7 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9HAB3 Human |
| Alternative names: | GPR172A hRFT3 PAR1 PERV-A receptor 1 Porcine endogenous retrovirus A receptor 1 Protein GPR172A RFT3 RFT3_HUMAN Riboflavin transporter 3 |
|
Fig1:
Western blot analysis of SLC52A2 on different lysates with Rabbit anti-SLC52A2 antibody (HA500611) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: A549 cell lysate (no heat) Lane 3: HeLa cell lysate Lane 4: HeLa cell lysate (no heat) Lane 5: MCF7 cell lysate Lane 6: MCF7 cell lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500611) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |