SLC52A2 Rabbit Polyclonal Antibody
cat.: HA500611
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human SLC52A2 aa 201-250.
Positive control: A549 cell lysate, HeLa cell lysate, MCF7 cell lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:5,000
Uniprot #: SwissProt: Q9HAB3 Human
Alternative names: GPR172A hRFT3 PAR1 PERV-A receptor 1 Porcine endogenous retrovirus A receptor 1 Protein GPR172A RFT3 RFT3_HUMAN Riboflavin transporter 3
Images
HA500611_1.jpg Fig1: Western blot analysis of SLC52A2 on different lysates with Rabbit anti-SLC52A2 antibody (HA500611) at 1/5,000 dilution.

Lane 1: A549 cell lysate
Lane 2: A549 cell lysate (no heat)
Lane 3: HeLa cell lysate
Lane 4: HeLa cell lysate (no heat)
Lane 5: MCF7 cell lysate
Lane 6: MCF7 cell lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500611) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.