CD38 Rabbit Polyclonal Antibody
cat.: HA500613
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within mouse CD38 aa 1-304. |
| Positive control: | Rat colon tissue lysate, Rat spleen tissue lysate, Mouse spleen tissue lysate, mouse splenocytes. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:5,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P56528 Mouse | Q64244 Rat |
| Alternative names: | Acute lymphoblastic leukemia cells antigen CD38 ADP ribosyl cyclase 1 ADP ribosyl cyclase ADP ribosyl cyclase/cyclic ADP-ribose hydrolase ADP-ribosyl cyclase 1 ADPRC 1 ADPRC1 cADPr hydrolase 1 CD 38 CD38 CD38 antigen (p45) CD38 antigen CD38 molecule Cd38-rs1 CD38_HUMAN CD38H Cyclic ADP ribose hydrolase Cyclic ADP ribose hydrolase 1 Cyclic ADP-ribose hydrolase 1 EC 3.2.2.5 Ecto nicotinamide adenine dinucleotide glycohydrolase I-19 I19 (mouse) Lymphocyte differentiation antigen CD38 NAD(+) nucleosidase NIM-R5 antigen NIMR5 antigen (mouse) OTTHUMP00000158633 OTTHUMP00000217743 p45 T10 |
Images
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Fig1:
Western blot analysis of CD38 on different lysates with Rabbit anti-CD38 antibody (HA500613) at 1/5,000 dilution. Lane 1: Rat colon tissue lysate Lane 2: Rat spleen tissue lysate Lane 3: Mouse spleen tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: Lane 1: 2 minutes; Lane 2-3: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500613) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of mouse splenocytes labeling CD38 with Rabbit anti-CD38 antibody (HA500613) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (HA500613) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Flow cytometric analysis of mouse splenocytes labeling CD38. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA500613, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.