HTR3A Rabbit Polyclonal Antibody
cat.: HA500614
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 2ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 56 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse HTR3A aa 51-100.
Positive control: Mouse brain tissue lysate, Rat brain tissue lysate.
Subcellular location: Postsynaptic cell membrane, Cell membrane.
Recommended Dilutions:
  WB

1:2,000
Uniprot #: SwissProt: P23979 Mouse | P35563 Rat
Alternative names: 5 HT 3 5 HT3 receptor 5 HT3A 5 HT3A receptor 5 HT3RA 5 hydroxytryptamine (serotonin) receptor 3A 5-HT-3 5-HT3-A 5-HT3A 5-HT3R 5-hydroxytryptamine receptor 3 5-hydroxytryptamine receptor 3A 5HT receptor 5HT3 serotonin receptor 5HT3A_HUMAN 5HT3R HTR3 Htr3a Serotonin receptor 3A Serotonin-gated ion channel receptor 3a Serotonin-gated ion channel receptor
Images
HA500614_1.jpg Fig1: Western blot analysis of HTR3A on different lysates with Rabbit anti-HTR3A antibody (HA500614) at 1/2,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 56 kDa
Observed band size: 56 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500614) at 1/2,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.