| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse HTR3A aa 51-100. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Postsynaptic cell membrane, Cell membrane. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P23979 Mouse | P35563 Rat |
| Alternative names: | 5 HT 3 5 HT3 receptor 5 HT3A 5 HT3A receptor 5 HT3RA 5 hydroxytryptamine (serotonin) receptor 3A 5-HT-3 5-HT3-A 5-HT3A 5-HT3R 5-hydroxytryptamine receptor 3 5-hydroxytryptamine receptor 3A 5HT receptor 5HT3 serotonin receptor 5HT3A_HUMAN 5HT3R HTR3 Htr3a Serotonin receptor 3A Serotonin-gated ion channel receptor 3a Serotonin-gated ion channel receptor |
|
Fig1:
Western blot analysis of HTR3A on different lysates with Rabbit anti-HTR3A antibody (HA500614) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 56 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500614) at 1/2,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |